000			02097nam a22001817a 4500
003			OSt
005			20250328141324.0
008			250328b |||||||| |||| 00| 0 eng d
040			_cNVSU
100			_aVisaya, Janus Drake D.
245			_aIn vitro antibacterial efficacy of virgin coconut oil prepared through traditional boiling method against staphylococcus aureus _cJanus Drake D. Visaya
260			_aBayombong: _bNueva Vizcaya State University, _c2022
300			_aix, 34 leaves : _bphotos ; _c28 cm.
500			_aThe study was conducted to determine the efficacy of Virgin coconut oil prepared through traditional boiling method in in vitro experiment. Using the agar dilution method. the experiment has six (6) treatments and was laid out in replication design Treatment one (T1) is the positive control (streptomycin), treatment two (T2) is the negative control which was pure MHA, treatment three (T3) for 100 µl VCO, treatment four (14) was 200 µl VCO, treatment five (T5) was 300µl, and treatment six (T6) was 400 µl of VCO A total of 18 MHA tubes were used and put into a test tube rack. Result showed that the volume of 100, 200, 300, and 400 µl of 100% VCO showed no growth of bacteria at 18 hours of Incubation. At 24 hours of incubation, there are bacterial colonies but not easily recognized A1 48 hours of incubation, all of the treatments have well-formed bacterial colonies except for the positive control. This VCO prepared through the traditional boiling method which is predominantly composed of lauric acid has inhibitory action on the growth of Staphylococcus aureus within 18 hours of incubation. It is recommended to the future researcher the use of VCO in in vitro experiment to the bacteria such as Escherichia coli using agar dilution method, use in vivo experiment in an animal infected with Staphylococcus aureus and compare the topical and oral efficacy of VCO against Staphylococcus aureus in vivo experiments.
500			_aThesis (Doctor of Veterinary Medicine)
504			_aincludes appendices and bibliographical references.
942			_2ddc _cU _n0
999			_c461 _d461