000 02867nam a22001817a 4500
003 OSt
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008 250321b |||||||| |||| 00| 0 eng d
040 _cNVSU
100 _aDe Leon, Kristine Jane P.
245 _aExtrinsic motivation and performance of teachers in schools division of Nueva Vizcaya/
_cKristine Jane P. De Leon
260 _aBayombong:
_bNueva Vizcaya State University,
_c2023
300 _axiii, 119 leaves :
_billustrations (some colored), photos ;
_c28 cm.
500 _a A paper-based microfluidic bioanalytical tool was developed and evaluated for its performance and effectiveness as a tool in science teaching. This innovative device is engineered to detect Ochratoxin A (OTA) in food and food sources by leveraging a colorimetric signal generated through enzymatic redox reactions within an integrated assay system. During its development, utmost consideration was paid in optimizing critical parameters influencing functionality and operation, including the fine-tuning of printing and detection mechanisms. In performance evaluations, the device exhibited a remarkable ability to detect OTA in whole wheat grain samples, achieving a limit of detection (LOD) as low as 512.30 ppb. Further investigation of the developed device confirmed its selectivity for OTA even in the presence of other mycotoxins. It demonstrated a high sensitivity to small changes in concentration, consistently yielding precise signal generation and highly accurate results. These attributes make the device a promising tool for commercial applications, particularly in the detection of OTA in animal food products. The performance and effectiveness of the tool in science teaching was evaluated through a post-activity survey administered to selected participants who engaged in a laboratory exercise employing the developed device. The activity was designed to introduce the concept of chemical analysis, aligning with the principles of Analytical Chemistry. It served as a culmination of the students comprehension of chemistry, specifically focusing on topics such as stoichiometry, dilution, and redox reactions. Each respondent completed a questionnaire structured around four key dimensions of the teaching and learning process: goals and objectives, content, motivation and participation, and organization and structure. The survey findings revealed that the majority of students regarded the device very highly effective in its performance as a tool in science teaching. Their firsthand experience on the use of the device not only piqued their interest but also empowered them to actively engage in discussions and hand-on aspects of the learning process.
500 _aThesis (Master of Education in Science Education - General Science)
504 _aIncludes appendices and bibliographical references
942 _2ddc
_cTH
_n0
999 _c365
_d365