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Predaceous activity and efficacy of nematophagous fungi (arthrobotry oligospora) on the larva of common housefly (mus domestica)/ Noverlee P. Calub

By: Material type: TextTextPublication details: Bayombong: Nueva Vizcaya State University, 2013.Description: xviii, 47 leaves : illustrations, photos ; 28 cm
Item type: Undergraduate Research
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Undergraduate Research Nueva Vizcaya State University - Bayombong Campus General Reference Section U (DVM) C166p 2013 (Browse shelf(Opens below)) Not for loan U538

The study was conducted in order to determine the predaceous activity and efficacy of Arthrobotrys oligospora on the larva of common housefly (Musca domestica). Specifically, the study aimed to compare the larvicidal effect of A. oligospora using different concentrations against commercially prepared larvicides (malathion™) and to determine the mode of action in parasitism of A oligospora on the larva of M. domestica using Lethal Dose 50 (LD50) through microscopic examination every three (3) hours interval.

Synchronized reproduction of adult common housefly to produce similar stage of 1 larva instar was done. A total of 250 larvae were used in the study wherein 10 larvae were used for each treatment. There were five treatments, each treatment was replicated five times as follows: 11- Malathion (positive control), T2 Distilled Water (negative control), T3-2000 spores of NF, T4-4000 spores of NF, TS-6000 spores NF.

The study showed and proved that nematophagous fungi is comparable with commercially prepared larvicide (malathion) in terms of percentage mortality starting 36 hours until 48 hours exposure. Therefore, it is effective as biological control against larva of common housefly. Based on the result of the study, nematophagous fungi has the ability to kill larva of the common housefly by causing entrapment that led to stress, inability to move and eat until thin hyphae penetrate the cuticle of larva which swells to become infection bulbs and cause it to undergo lysis. Nematophagous fungi is recommended as alternative to commercially prepared insecticide because of its comparable effect. It is also recommended that the next researcher will conduct the following: practical application of nematophagous fungi on chicken feces using alkalinize to offset the acidity of feces; conduct histopathological exam in evaluating larvicidal effect on nematophagous fungi; use other alternative culture medium for A. oligospora aside from Potato Dextrose Agar anduse other method of application of treatments.

Thesis (Doctor of Veterinary Medicine)

Includes appendices and bibliographical references.

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