Antimicrobial effect of patani (phaseolus lunatus linn.) leaf extract against staphyloccus aureus (ATTC 25923) and escherichia coli (NRRL B-3709) in vitro/ (Record no. 737)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02956nam a22001817a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250402150528.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 250402b |||||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Transcribing agency | NVSU |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Talapi, Oro D. |
| 245 ## - TITLE STATEMENT | |
| Title | Antimicrobial effect of patani (phaseolus lunatus linn.) leaf extract against staphyloccus aureus (ATTC 25923) and escherichia coli (NRRL B-3709) in vitro/ |
| Statement of responsibility, etc. | Oro D. Talapi |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Bayombong: |
| Name of publisher, distributor, etc. | Nueva Vizcaya State University, |
| Date of publication, distribution, etc. | 2012. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | xi, 37 leaves : |
| Other physical details | illustrations, photos ; |
| Dimensions | 28 cm. |
| 500 ## - GENERAL NOTE | |
| General note | The study compared the antibacterial effect of crude Patani leaf extract (PLE) with commercially available antibiotics (Penicillin G) and (Streptomycin) and identified the most effective concentration capable of inhibiting the growth of Staphylococcus aureus and Escherichia coli It was laid out following the Factorial in Completely Randomized Design with five treatments replicated three times.<br/><br/>The study was conducted in two sets: Staphylococcus aureus was used as the test organism with (Peniciilin G) as control. The treatments were designated as follows: Treatment 1 (Pen G), Treatment 2 (25% PLE), Treatment 3 (50% PLE), Treatment 4 (75% PLE), and Treatment 5 (100% PLE). The second set used (Streptomycin) as control and Escherichia coli as the test organism with the used of Patani leaf extract in varying doses of concentrations as follows: 25%, 50%, 75%, and 100%. <br/><br/>Zone of inhibition after 12 hours of incubation of Staphylococcus aureus showed that T3, T4, T5 exhibited moderate antibacterial efficacy and are comparable to each other. No zone of inhibition noted in T2 (25% Leaf extract) throughout the 36-hour duration of the study. After 24 to 36 hours of incubation T3 (50% Leaf extract) continue to have better degree of antimicrobial efficacy as compared with that of T4 and T5. The descending zone of inhibition obtained from T4, and T5 might be due to the difficulty ofthe crude extract to diffuse through the agar. <br/><br/>In the study using the Escherichia coli, patani leaf extract at different concentrations after varying hours of incubation did not exhibit any significant effect on the bacterial growth as the Escherichia coli colonies formed around close to the patani leaf extract sample disks. No zones of inhibition were observed in these treatments except for the positive control. <br/><br/>The result of the study revealed a certain degree of antimicrobial efficacy of patani leaf extract against Staphylococcus aureus but not against gram negative Escherichia coli. Fifty percent (50%) dilution of the patani extract proved to be the optimum concentration that best inhibits staphylococci growth using agar diffusion test. However, it is not comparable to that observed in the commercially available antibiotic positive control treatment. |
| 500 ## - GENERAL NOTE | |
| General note | Thesis (Doctor of Veterinary Medicine) |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes appendices and bibliographical references. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Undergraduate Research |
| Suppress in OPAC | No |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Source of acquisition | Total checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | Nueva Vizcaya State University - Bayombong Campus | Nueva Vizcaya State University - Bayombong Campus | General Reference Section | 04/02/2025 | Donation | U (DVM) T137a 2012 | U487 | 04/02/2025 | 04/02/2025 | Undergraduate Research |
